منابع مشابه
Tips and Tricks in Fluorescence In-situ Hybridization (FISH)- based Preimplantation Genetic Diagnosis /Screening (PGD/PGS)
As numerical and structural defects in chromosomes are an inevitable consequence of IVF, Pre-implantation genetic diagnosis and screening (PGD/PGS) methods are used for detecting abnormalities in embryos before implantation to the uterus to increase the successful rate of IVF. Pre-implantation genetic diagnosis and screening approaches can be achieved by different techniques such as NGS, CGH an...
متن کاملMultiplexed in situ hybridization using hybridization chain reaction.
CHOI ET AL. l DESCRIBE PROGRAMMABLE IN SITU AMPLIFIERS based on the mechanism of hybridization chain reaction (HCR) that enable simultaneous mapping of multiple target mRNAs within whole-mount zebrafish embryos. In contrast to traditional in situ hybridization approaches, HCR in situ amplification is performed for all target mRNAs simultaneously, so the experimental timeline is independent of t...
متن کاملDifferentiating and Categorizing of Liposarcoma and Synovial Sarcoma Neoplasms by Fluorescence in Situ Hybridization
Background & Objective: Soft tissue sarcomas (STS) constitute an uncommon and heterogeneous group of tumors of mesenchymal origin and various cytogenetic abnormalities ranging from distinct genomic rearrangements, such as chromosomal translocations and amplifications, to more intricate rearrangements involving multiple chromosomes. Fluorescence in situ...
متن کاملRetrospective assessment of radiation dose by Fluorescence In Situ Hybridization and evaluation of stable chromosomal aberrations
Estimation of absorbed dose for radiation workers or person involved in different radiological accident is the aim of biodosimetry. Cytogenetic methods are the most current and applicable biodosimetry tools. In chronic or protracted exposure, fluorescence in situ hybridization (FISH), stable chromosomal aberration is used for estimation of absorbed dose. For precise estimation of absorbed dose,...
متن کاملHighly multiplexed subcellular RNA sequencing in situ.
Understanding the spatial organization of gene expression with single-nucleotide resolution requires localizing the sequences of expressed RNA transcripts within a cell in situ. Here, we describe fluorescent in situ RNA sequencing (FISSEQ), in which stably cross-linked complementary DNA (cDNA) amplicons are sequenced within a biological sample. Using 30-base reads from 8102 genes in situ, we ex...
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ژورنال
عنوان ژورنال: The Journal of Molecular Diagnostics
سال: 2019
ISSN: 1525-1578
DOI: 10.1016/j.jmoldx.2019.01.010